bv伟德源自英国始于1946(中国)有限公司

当前位置：首页 > 试剂及试剂盒 >其他 >Signagen试剂 > LipoJet™ (Ver. II) DNA In Vitro Transfection Reagent

LipoJet™ (Ver. II) DNA In Vitro Transfection Reagent
￥2400.00

品牌： Signagen

规格：

1.0 ml

货期：

现货

货号：

SL100468

在线咨询

商品详情

产品介绍：
利用我们的创新和专有的脂质体共轭合成技术，LipoJet™转染试剂盒，运用了新型的含氟阳离子脂质体配方，同市场上的其他脂质体转染试剂有着显著的区别，其特点是转染效率高、毒性小。LipoJet™转染试剂盒是最强大的基因转导工具，适于几乎有所有体外基因转导的应用，包括质粒DNA、siRNA和shRNA转染和DNA/siRNA共转等。
试剂盒内容：
- LipoJet™ 试剂，1.0ml，足够用1000次DNA的转染(24孔板中0.5 ug DNA/孔)足够用1000次siRNA转染(24孔板中10 nM siRNA/孔)。
- LipoJet™转染缓冲液(5X )，按照最大化的转染效率配制，8.0ml浓缩液就可以制成40ml的工作液体。
产品特点：
- 适用于广泛的哺乳动物细胞
- 极高效率地转染DNA，siRNA和DNA/siRNA共转染
- 10 nM siRNA能达到95%的沉默效应
- 无血清和抗生素干扰
- 简单易用的操作步骤
- 适用于高通量的应用
- 细胞毒性最低
储存条件：
4⁰C储存。若储藏合适，产品的稳定性能保持12个月以上。
LipoJet™体外转染试剂具有极广谱的转染活性。

细胞类型	DNA转染效率	siRNA转染效率
293, 293T 3T3, NIH 3T3 3T3-L1 A549 B16-F10 BNLCL.2 C2C12 C3H/10T1/2 Caco-2 Rat primary cardiomyocytes CHO COS1 COS7 Human bladder carcinomal cell mES iMEF HBEC HCT116 Hela HepG2 HT-29 HuH-7 LNCaP MC3T3-E1 MCF10A MCF7 MDA-MB-231 MDCK MEF Human primary melanocytes Human primary melanoma Human immortalized microglial mIMCD-3 MRC-5 N2A PC3 RAW 264.7 RPE SH-SY-5Y SK-OV-3 U-2OS VERO	85% - 30% 80% 75% 55% 80% 60% 20% 10% 80% 70% 75% 80% 50% 55% 45% 75% 80% 60% 45% 45% 45% 50% 40% 50% 50% 20% 50% 50% 50% 50% 50% 50% 68% 70% 45% 30% 75% 70% 80% 40%	- 90% at 20 nM - 90% at 20 nM - - 90% at 40 nM - 80 % at 30 nM - - - - - - - - - - - - - - - - - - - 85% at 30 nM 85% at 30 nM - - - - - - - - 90% at 30 nM - - -

以下图例表明LipoJet™体外转染试剂盒出色的DNA和siRNA转染效率。
LipoJet Transfection Reagent
A efficiency comparison of LipoJet™ reagent vs. brand name products to transfect Hela, Cos-7, NIH-3T3, CHO and HEK293 (left panel) and Cos-7 (right panel).  pEGFP-N3 cDNA (0.5 µg/well of 24-well plate) was transfected into different mammalian cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers. The transfection efficiency were analyzed via FACS 48 hours post transfection.
LipoJet_Cell_Viability
A toxicity comparison of LipoJet™ reagent vs. brand name products to transfect Hela cells.  pEGFP-N3 cDNA (1.0 µg/well of 24-well plate) was transfected to Hela cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers. The MTT assay (right panel) and phase contrast imaging (left panel) were used to analyze the cell viability 48 hours post transfection.
LipoJet_LIPO2000_POLYJET_Comparison_HEK293
A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on HEK293T cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into 293T cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet™/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.
LipoJet_LIPO2000_POLYJET_Comparison_HELA
A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on Hela cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into Hela cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet™/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

LipoJet™试剂介导的DNA/siRNA共转染表现出色的基因沉默效应。分别由LipoJet™试剂转染GFP的cDNA (左图, 0.25µg/孔，24孔板)作为对照和共转染GFP的cDNA (0.25µg/孔，24孔板)/GFP的siRNA (终浓度10 nM，右图)至HEK293细胞 (上图), Hela细胞(中图)和SaoS-2细胞(下图)。转染24小时后，使用尼康Eclipse荧光显微镜检查GFP荧光。结果显示，GFP的cDNA和GFP的siRNA共转染导致GFP的表达被显著抑制。

LipoJet_HUVEC_mCherry-FITC-siRNA
Exceptional DNA/siRNA co-transfection efficiency on HUVEC. Co-transfection of mCherry cDNA (0.10 µg per well of 24-well plate) and FITC conjugated siRNA (final 30 nM per well of 24-well plate) to HUVEC with LipoJet™ reagent gave rise to 60% mCherry+ (phase contrast overlapped with mCherry imaging, left panel) and nearly 100% FITC-siRNA+ (phase contrast overlapped with FITC imaging, right panel) HUVEC 24 hours after transfection. The pictures were given from Dr. Pan Kong of USC as courtesy.
LipoJet_EG5_HEK293
LipoJet_EG5_HELA
Excellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 (upper panel) and Hela (lower panel) cells with LipoJet™ reagent at 10 nM EG5 siRNA. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 10 nM) to HEK293 and Hela cells, leading to more than 80% of "round-up" phenotype of HEK293 and Hela cells 24h post transfection over negative control (final 10 nM with sham EG5 siRNA). The phenotype of "rounded-up" HEK293 and Hela cells were visualized 24h post transfection with a Nikon microscope.