- 欢迎来到bv伟德源自英国始于1946
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商品分类
010-64814275
010-64814275
| Description | |
|---|---|
| Overview | Serine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK. |
| Catalogue Number | 539480 |
| Brand Family | Calbiochem® |
| References | |
|---|---|
| References | Watazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81. Lebherz, H.G., et al. 1986. Biochem. J. 233, 51. Ebeling, W., et al. 1974. Eur. J. Biochem. 47, 91. |
| Product Information | |
|---|---|
| CAS number | 39450-01-6 |
| Activity | ≥30 mAnsonU/mg dry weight |
| Unit of Definition | One mAnson unit is defined as the amount of enzyme that will liberate 1.0 µmol of Folin-positive amino acids (calculated as tyrosine) per min at 25°C, pH 7.5. |
| EC number | 3.4.21.14 |
| Form | White lyophilized solid |
| Formulation | Lyophilized from calcium acetate |
| Biological Information | |
|---|---|
| Specific Activity | ≥40 mAnsonU/mg protein |
| Physicochemical Information | |
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| Contaminants | DNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C |
| Storage and Shipping Information | |
|---|---|
| Ship Code | Ambient Temperature Only |
| Toxicity | Irritant |
| Storage | +2°C to +8°C |
| Do not freeze | Ok to freeze |
| Special Instructions | Following reconstitution, store in the refrigerator (4°C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4°C. |
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