描述
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® DPBS formulations for a range of cell culture applications.
This DPBS is modified as follows:
Without
Calcium
Magnesium
Phenol Red
The complete formulation is available.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DPBS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® DPBS product made in our Scotland facility (14190-094). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. ">protocol for details).
规格
Form: | Liquid |
Inorganic Salts: | No Calcium, No Magnesium |
Concentrated: | 1× |
Phenol Red Indicator: | No Phenol Red |
pH Range: | 7.0 - 7.3 |
Osmolality: | 270 - 300 mOsm/kg |
Product Size: | 500 mL |
Classification: | Animal Origin-Free |
Endotoxin Level: | 0.00 - 0.50 EU/ml |
Sodium Pyruvate Additive: | No Sodium Pyruvate |
Green Features: | Sustainable packaging |
Shipping Condition: | Room Temperature |
内容及储存
Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 36 months from date of manufacture
培养基配方
14190 - DPBS, no calcium, no magnesium
Components | Molecular Weight | Concentration (mg/L) | mM |
Inorganic Salts | |||
Potassium Chloride (KCl) | 75 | 200 | 2.6666667 |
Potassium Phosphate monobasic (KH2PO4) | 136 | 200 | 1.4705882 |
Sodium Chloride (NaCl) | 58 | 8000 | 137.93103 |
Sodium Phosphate dibasic (Na2HPO4-7H2O) | 268 | 2160 | 8.059702 |
C14190500BT- DPBS, no calcium, no magnesium-MSDS