Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Quality
• Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
• Tested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations are determined by Lowry method
• Endotoxin units are measured by Limulus Amoebocyte Lysate assay
• BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0 mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor
Source
Engelbreth-Holm-Swarm Mouse Tumor
Application
• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• In Vitro and In Vivo Angiogenesis Assays
• In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
Features
• Certified LDEV-Free
• Extensive quality control exceeding industry standard
• Rigorously validated for functionality
• Most extensively references basement membrane matrix
Qty./Pk.: | 1 |
Qty./CS.: | 5 |
Size: | 5 x 10 mL |
Shipping: | Refrigerated Shipping DI |