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Alexa Fluor® 647 Mouse Anti-Human CD107b  Clone  H4B4  (RUO)

• Brand

• BD Pharmingen™

• Alternative Name

• LAMP-2; LAMP2; LAMPB; LGP110

• Vol. Per Test

• 5 µl

• Isotype

• Mouse IgG1, κ

• Reactivity

• Human (QC Testing) 

• Application

• Intracellular staining (flow cytometry) (Routinely Tested) 
  

• Immunogen

• Human Adult Adherent Peripheral Blood Cells

• Workshop No.

• V P007

• Storage Buffer

• Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

DESCRIPTION

The H4B4 monoclonal antibody specifically binds to CD107b. CD107b is a heavily glycosylated, ~120 kDa type I transmembrane protein that is also known as lysosomal-associated membrane protein 2 (LAMP-2/LAMP2), LAMPB, or LGP110. CD107b is expressed in the lysosomal and endosomal membranes of most cell types where it may play a role in maintaining membrane integrity. CD107b is also expressed on the surface of activated platelets and lymphocytes, including T lymphocytes, e.g., degranulating CD8+ cytotoxic T cells. CD107b serves as a ligand for selectins, including E-selectin, and mediates cellular adhesion. CD107a/ LAMP-1 and CD107b /LAMP-2 are carriers for poly-N-acetyllactosamines, and display the sialyl Le[x] antigen. CD107b is likewise expressed on some tumor cells and cell lines, including U937 and KG1a, and may facilitate tumor metastases.

FORMAT

• Format

• Alexa Fluor® 647

• Excitation Source

• Red 633 nm

• Excitation Max

• 650 nm

• Emission Max

• 668 nm

Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.