HiTrap SP Sepharose FF

HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

• Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.

• The industry standard for ion exchange chromatography.

• High flow rates and good scale-up potential.

• Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.

• Predictable scale-up

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a resin with higher porosity, which is particularly useful for the purification of high molecular mass proteins.